By Phyllis R. Brown, Eli Grushka, Susan Lunte

ISBN-10: 020399695X

ISBN-13: 9780203996959

ISBN-10: 0824753410

ISBN-13: 9780824753412

That includes trustworthy, up to the moment reports of significant advancements in chromatography, quantity forty three reports the newest advances within the box with contributions and present learn from world-renowned leaders in undefined. It offers particular discussions of present subject matters, matters, and advancements in separation technology and analyzing issues resembling gradient elution in liquid column chromatography and strong part microextraction.

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Extra info for Advances In Chromatography: Volume 43 (Advances in Chromatography)

Example text

The calculation of the elution times or of the elution volumes by integration of Eq. (5a,b) in ‘‘ideal gradient elution’’ is based on several simplified assumptions concerning both the phase equilibrium in the column and the function of the instrumentation used. 1. The interactions between various compounds in the separation column should not change the column properties and the gradient profile should not change as it moves along the column, so that its profile does not change from the start of the gradient till the elution of the last sample solute.

4,26,27]. Theoretically, it should be possible to compensate for any nonlinear dependence of log k on u by designing an appropriate complementary gradient profile, but setting a suitable program for an exactly linear change in log k during the gradient elution can be impractically tedious and often is not feasible with many commercial instruments. An easier approach, which can be used for a great variety of combinations of gradient programs and chromatographic phase systems, divides the function characterizing changes in k with increasing V (or t) into two partial contributions [2,3,54] 1.

Equation (8) neglects an additional band compression in gradient elution resulting from a faster migration of the trailing edge of the band in a mobile phase with a higher elution strength, whereas the leading edge moves along the column more slowly. In exact calcu- Gradient Elution in LC Chromatography / 19 lations, the bandwidths calculated using Eq. (8) can be corrected by a band compression factor, G [73,75,76]. The effect of the additional band compression in most cases results in approximately 10% reduction of the experimental wg [4,23,77].

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Advances In Chromatography: Volume 43 (Advances in Chromatography) by Phyllis R. Brown, Eli Grushka, Susan Lunte


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